Cell-Induced Stress
세포가 낳은 고통
As a much-hailed breakthrough in stem-cell science unravelled this year, many have been asking: ‘Where were the safeguards?’
줄기세포과학에서의 많이 회자되는 돌파구가 올해 드러남에 따라, 많은 사람들은 ‘안전장치가 있었나?’라는 의문을 제기하고 있다.
It seemed almost too good to be true — and it was.
그것은 거의 너무나 반가워 사실이 아닐 것 같았지만 사실이었다.
Two papers1, 2 that offered a major breakthrough in stem-cell biology were retracted on 2 July, mired in a controversy that has damaged the reputation of several Japanese researchers.
줄기세포 생물학에서의 주요한 돌파구를 제공한 두 편의 논문 1, 2는 7월 2일에 철회되었고, 여러 일본 연구원들의 명예를 손상시킨 논란의 수렁으로 빠졌다.
For scientists worldwide it has triggered painful memories of a decade-old scandal.
전 세계 과학자들에게 이것은 10년 전 스캔들의 고통스런 기억을 떠올리게 한다.
In February 2004, South Korean researcher Woo Suk Hwang announced that he had generated stem-cell lines from cloned human embryos, creating a potential source of versatile, therapeutic cells that would be genetically matched to any patient.
2004년 2월, 한국의 황우석 연구원은 복제된 인간배아에서 줄기세포를 만들었고 유전적으로 어떠한 환자에게도 맞는 다용도의 치료세포의 잠재적 근원을 만들었다고 발표했다.
A frenzy of excitement followed this and a subsequent publication, but that didn’t compare with the media firestorm when the results were revealed to be fabricated.
엄청난 흥분과 추후의 출판가 뒤따랐지만, 결과가 조작되었던 것이라 발표되었을 때 그것은 언론의 비난과는 비교되진 않았다.
firestorm:
The two main cloning papers were retracted, and the careers of some dozen scientists were devastated.
두 편의 주요논문은 철회되었고 여러 과학자들의 경력은 치명적 손상을 입었다.
In the soul-searching that followed, ‘research integrity’ became a hot topic, scientists re-evaluated the responsibilities of authorship, and institutions vowed to improve the way that they police their staff.
자신의 성찰이 뒤따라, 연구청렴성이 주요 쟁점이 되었고, 과학자들은 저작자의 책임을 재평가하였고, 연구소는 자신들의 직원을 감독하는 방식을 개선하기로 다짐했다.
soul-searching : 자기탐구(분석)
Nature and other journals also made promises, saying that they would vet manuscripts more thoroughly.
네이처와 다른 학술지들도 더욱 철저히 원고를 심사할 것이라고 또한 다짐을 했다.
vet : 조사[심사]하다
In an Editorial at the time, Nature wrote: “Keeping in mind the principle that extraordinary claims require extraordinary proof, Nature may in rare cases demand it.”
그 당시 네이처지의 한 사설은 경이로운 주장은 그에 걸맞은 증거를 요구한다는 원칙에 입각하여 네이처는 그것을 요구할 가능성이 있다는 글의 실었다.
A year later, when Shoukhrat Mitalipov of the Oregon Health & Science University in Portland claimed to have cloned embryonic-stem-cell lines from monkeys, Nature required independent tests to verify that the lines came from the monkey donors.
1년이 지나, 포틀랜드의 오레곤 보건과학대학의 Shoukhrat Mitalipov가 원숭이로부터 배아줄기세포선 복제에 성공했다고 주장했을 때, 네이처는 그 세포선이 원숭이 기부자로부터 온 것인지를 입증할 독자적인 실험을 요구했다.
This verification was published alongside the cloning paper.
이 입증실험은 복제논문과 함께 실렸다.
“I applaud what they did,” says Alan Trounson, the outgoing president of the California Institute for Regenerative Medicine in San Francisco, who helped with the testing.
샌프란시스코의 재생약품 캘리포니아 연구소에서 그 실험에 도움을 준 물러나는 연구소장 Alan Trounson는 “그들이 행한 것에 찬사를 보낸다.”고 말하였다.
Then came Japan’s stem-cell case.
그 이후 일본의 줄기세포 문제가 야기되었다.
This January, Haruko Obokata, a young biochemist at the RIKEN Center for Developmental Biology (CDB) in Kobe, Japan, reported in Nature that she had converted mouse cells to an embryonic-like state merely by subjecting them to stress, such as physical pressure or exposure to acid.
일본 고베의 발생생물학 센터의 젊은 생화학자인 Haruko Obokata는 네이처에 생쥐세포를 물리적인 압력이나 산성에 노출하는 것과 같이 단순히 스트레스를 주어 배아와 같은 상태로 전환하였다고 보고하였다.
The process, labelled stimulus-triggered acquisition of pluripotency (STAP), was so contrary to current thinking that some scientists said they accepted it based only on the reputation of Obokata’s co-authors, who were some of the most trusted names in stem-cell research and cloning.
자극에 의한 전분화능 획득이라 명명된 그 과정은 현재의 생각과 너무나 대조되어 과학자들은 줄기세포 연구와 복제에서 가장 신임하는 Obokata의 공동저자들의 명예에 단지 근거하여 그들은 그것을 수용했다고 말했다.
pluripotency : (생명과학) 전분화능(前分化能)
But the paper that set out the fundamental technique was soon shot full of holes. There was plagiarized text in the article.
기초기술로 시작한 논문은 허점투성이로 엉망이었다. 그 논문에는 표절된 구절이 있었다.
shot full of holes : 허점투성이로 엉망인 / plagiarize : 표절하다
Figures showed signs of manipulation, and some images were identical or nearly identical to those used later in the same paper and elsewhere to represent different experiments.
More damning were genetic analyses that strongly suggested the cells were not what they were purported to be.
And although deriving STAP cells was advertised as simple and straightforward, no one has yet been able to repeat the experiment.
교재는 서점에서 구입하실 수 있습니다.
Within the space of six months, Obokata was found guilty of misconduct by her institution; well-respected scientists, including RIKEN head Ryoji Noyori, bowed their heads in apology; and both papers were retracted9. In the end, the evidence for STAP cells seemed so flimsy that observers began to ask where were the extra precautions and the ‘extraordinary proof’ that had been promised post-Hwang.
The case has reopened difficult questions about the quality of research and peer review, and the responsibilities of co-authors, institutions and journals. It is also making its mark as an example of how not to do things. The episode has already become a “parable in my lab for teaching students about scientific ethics”, says Jeanne Loring, a stem-cell biologist at the Scripps Research Institute in La Jolla, California.
In this article, the news team at Nature — which is editorially independent from the journal team that reviewed and published the STAP papers — attempts to find out what went wrong and what can be learned from the case.
CAUGHT IN ISOLATION
The STAP saga has its roots in a contentious hypothesis more than a decade old. In 2001, Charles Vacanti, an anaesthesiologist at the Brigham and Women’s Hospital in Boston, Massachusetts, said that he had found “spore-like cells” in virtually every type of mammalian tissue10. According to Vacanti, these cells were pluripotent — that is, they could develop into any type of cell in the body — and seemed to lie dormant until activated, perhaps by injury or disease, to regenerate tissue.
Vacanti told Nature’s news team in January that by 2006 his laboratory could grow the cells in large numbers, but that they still “were not exceptionally well characterized by us”. That is, the team had not demonstrated pluripotency. This was a job he gave to Obokata, a graduate student who had joined his lab in 2008.
Proving pluripotency is often done by injecting cells into a developing mouse embryo — creating a ‘chimaera’ — and tracking their fate. It is a difficult experiment, and Obokata needed help. “I was looking for the god’s hand of chimaeric-mouse generation,” she said back in January. A Google search led her to famed mouse cloner Teruhiko Wakayama at the CDB, whose lab she entered in 2011 as a visiting professor. After hundreds of failures to get cells derived from adult mice to show up in chimaeras, she and Wakayama switched to newborn mice as the source of the cells — and the process worked.
By that point, both Vacanti and Obokata were convinced that the stress of the isolation process was creating the pluripotent cells. Obokata said that the idea had come to her while she was taking a bath and reflecting on the stress in her own life.
In the experiments at RIKEN, she used acid to stress spleen cells from newborn mice, and she carried out further experiments to characterize their conversion with Yoshiki Sasai and Hitoshi Niwa, two highly regarded stem-cell biologists at the CDB. With the two key characteristics of STAP cells now demonstrated — they were pluripotent and were created using stressful conditions — she had enough data to publish two papers in Nature on 30 January1,2.
Obokata became an instant celebrity in Japan, where the media picked up on details such as the ‘Moomin’ cartoon stickers on her lab equipment and the traditional Japanese cooking apron, given to her by her grandmother, that she wore instead of a lab coat.
But within weeks, anonymous observers began noting mistakes in the papers, including evidence of image manipulation, duplications and plagiarism (see go.nature.com/e4dwry).Researchers also started to report that they could not reproduce the supposedly simple experiment.
On 1 April, a RIKEN investigative committee concluded that Obokata had committed scientific misconduct. She maintained that the results were real, but one by one her co-authors withdrew support for the findings. In principle, Nature retracts articles only when all co-authors agree, although in rare cases papers can be retracted even if one or more of the authors dissent. In June, Obokata relented and agreed to retract both papers (see go.nature.com/wsfox5). She has not responded to multiple requests for interview since April. She has, however, been invited to participate — under surveillance — in ongoing efforts at RIKEN to verify the original findings.
Should the papers have been published in the first place? Critics have argued that many of the flaws could have been identified beforehand by Nature — the easiest, in theory, being a 17-line passage that was taken almost word for word from a 2005 paper11 by another group.
To detect signs of plagiarism, most journals use a service called CrossCheck. It can compare a submitted manuscript with some 40 million published articles from around 100,000 titles, looking for text matches.
Nature editors did use CrossCheck and did not find the match. But the journal from which the text was lifted, In Vitro Cellular & Developmental Biology — Animal, had not been indexed by the service at the time. “Although the databases are very large and growing, there are limitations,” explains Rachael Lammey, a product manager at CrossRef in Oxford, UK, which provides the CrossCheck service. Such misses get flagged “a couple times a year”, she says, but there is no way to know how many instances of plagiarism fall through the cracks.
Moreover, identifying the match probably would not have halted publication. Many instances of copied text do not constitute plagiarism and just require citation of the original source. Indeed, the RIKEN investigative committee concluded that the passage — a methodological description — should have cited the original, but that the failure to do so was not misconduct.
The committee was more vexed by instances of manipulated and duplicated images in the STAP papers. Obokata had spliced together gel lanes from different experiments to appear as one. And she had used an image of cells in a teratoma — a tumorous growth that includes multiple types of tissue — that had also appeared in her PhD dissertation. The captions indicated that the image was being used to represent different types of cell in each case. The committee judged that in both instances, although she might not have intended to mislead, she should have been “aware of the danger” and therefore found her guilty of misconduct. Obokata claimed that they were mistakes and has denied wrongdoing.
PICTURE IMPERFECT
Image manipulation and duplication within the same manuscript can be detected, and journals are increasingly checking for such problems. Jana Christopher analyses images in every manuscript before they are accepted by EMBO Press, a journal publisher based in Heidelberg, Germany. She uses a set of automated adjustments created for the image software Photoshop by the US Office of Research Integrity that change attributes such as contrast and colour to make manipulations easier to spot (ori.hhs.gov/actions).
At the behest of the chief editor of The EMBO Journal, Bernd Pulverer, Christopher ran tests on the STAP papers without knowing their background. She spotted three problems:
the gel manipulation that was ultimately attributed to misconduct, a seemingly innocent duplicated image mistake and a composite image of cell colonies most probably done to save space.
“The aberrations we saw are fairly typical,” says Pulverer, who reports that around 20% of scanned manuscripts have been found to have such issues since the journal started looking for them in 2011. The Journal of Cell Biology (JCB), published by Rockefeller University Press in New York, has been systematically scanning figures and images in all accepted papers since 2002 and finds about the same rate.
But the journals do not immediately consider a problematic image fraudulent. Spliced gel lanes, for example, are often attempts to present data more clearly and concisely. In most cases, these manipulations are done naively, to create a ‘prettier’, more informative image, says Pulverer. But, he says, “in cases where we don’t obtain plausible explanations and source data for the figure in question, we dig deeper”. At the JCB, acceptance is revoked for about 1% of papers, according to the journal’s executive editor, Liz Williams.
Such scanning methods are far from foolproof. Some worry that alerting authors to problems with their images allows would-be fraudsters to improve their forgeries. And although manipulated images might be easy to spot, it is harder to identify duplications, especially when they come from other articles. “Cross-literature comparisons would require very high-powered search algorithms and probably a supercomputer,” says Pulverer. “This has been discussed for a number of years but never moved forward.” In the STAP case, current image-checking procedures would not have caught the problem with the teratoma image — or several other problems with the main paper1 that surfaced under closer scrutiny.
Philip Campbell, editor-in-chief of Nature, says: “We have concluded that we and the referees could not have detected the problems that fatally undermined the papers.” But scientists and publishers say that catching even the less egregious mistakes raises alarm bells that, on further investigation, can lead to more serious problems being discovered.
Many say that the tests should be carried out on all papers. Christopher says that it takes about one-third of her working week to check all accepted manuscripts for the four journals published by EMBO Press. At Nature and the Nature research journals, papers are subjected to random spot-checking of images during the production process. Alice Henchley, a spokeswoman for Nature, says that the journal does not check the images in all papers because of limitations in resources, and that the STAP papers were not checked. But she adds that as one outcome of this episode, editors “have decided to increase the number of checks that we undertake on Nature’s papers. The exact number or proportion of papers that will be checked is still being decided.”
In the face of extraordinary claims, checking images and text is hardly sufficient to test the veracity of results. Independent genetic analyses12,13 proved that the world’s first sheep cloned from adult cells, Dolly, was genetically identical to the mammary-gland cells from which she was cloned. And Snuppy, created by Hwang’s lab, was similarly confirmed to be the first cloned dog14,15. Both verifications took place after publication of the initial results in order to quell debate, but such verification also could be done pre-publication, before a controversy arises.
Nature took that step in 2007, when it solicited an independent test of Mitalipov’s monkey stem-cell lines, which showed them to be true clones. Those tests required coordination between researchers who had to navigate restrictions on sending cells across borders. It “dragged on for months”, says Mitalipov. He subsequently chose another journal, Cell, to publish his next big stem-cell cloning paper16 — demonstrating the process using human cells — partly for that reason. That paper was published 12 days after acceptance. An anonymous critic pointed out mistakes in the final manuscript including image duplication and mislabelled figures, but the authors proved that they were harmless errors (see Nature http://doi.org/mnk; 2013).
Some researchers say that it would be best if collaborators on a project carried out more stringent verification tests of their own before submitting a paper — something that Wakayama now woefully acknowledges. In the STAP case, however, it is not immediately obvious how this could have been done. Unlike for sheep, dogs or primates, genetically identical mouse strains and matched embryonic-stem (ES)-cell lines are widely available, making it easy to provide samples that look roughly correct. “There is no way to distinguish, genetically, ES cells from STAP cells originating from the same strain,” says Rudolf Jaenisch, a stem-cell biologist at the Whitehead Institute for Biomedical Research in Cambridge, Massachusetts.
That said, post-hoc genetic analyses do seem to be unravelling the STAP riddle. In March, as problems with the papers mounted, Wakayama outsourced genetic sequencing of the purported STAP cells (see Nature http://doi.org/tf8; 2014). The work focused on a certain characteristic — the spot where a gene encoding a fluorescent protein inserts itself into the genome — to pinpoint the cells’ origin. The results, which Wakayama announced in June, showed them to be different from the mice that had supposedly been used to make them (see Nature http://doi.org/tf9; 2014).
A step beyond genetic verification would be independent replication of the experiments. That would probably put undue burden on the scientists asked to do the work. But Richard Behringer, a developmental biologist at the MD Anderson Cancer Center in Houston, Texas, says that asking authors directly whether more than one person in the lab has reproduced the results would be one way for the journal “to ensure that all data and images in the manuscript were solid”.
REPLICATION ISSUES
Nature does not disclose communications between editors and authors, but Campbell says that there were four independent groups on the two papers, and “it was our understanding that the work was independently replicated”. When questions first arose about the STAP cells, moreover, co-authors on the papers were adamant that they had overseen replication.
Sasai says that his lab observed the generation of STAP cells. But in fact, he had asked Obokata to replicate only the first part of the STAP process — the expression of a protein, Oct4 — which he recorded with live imaging. At the time, he says, he did not consider “the possibility of a gap between these cells and the derivation of STAP stem cells”.
Wakayama said that he “independently” produced STAP stem cells that looked exactly like ES cells — development beyond what Sasai witnessed — which convinced him that the process was solid. After problems first emerged, he told the Nature news team in an e-mail: “I succeeded at RIKEN independently, therefore, I know this result is absolutely true.” Looking back now, though, he realizes that his replication was not completely independent — Obokata was at his side during the entire experiment “and oversaw every step in the process”, he says. Because he moved to a new position at the University of Yamanashi soon after that test, he never characterized the cells and could not rule out the possibility that they had been switched or contaminated. The RIKEN investigative committee found that Sasai and Wakayama, although not involved in the misconduct, carried “heavy responsibility” for what happened.
The co-author who has most confused the issue of replication is Vacanti. Within a week of the STAP papers being published, he sent photos of what he claimed were human STAP cells to the magazine New Scientist. As others failed to replicate the STAP experiment, he told the Nature news team in mid-February: “There really shouldn’t be any difficulty. If I can do it, anyone should be able to do so.” In mid-March, he published online a list of tips for making STAP cells “regardless of the cell type being studied”. To date, however, he has produced no additional evidence that he has derived STAP cells in his laboratory. In a statement released on 2 July, Vacanti asserted that although he agreed to the retractions owing to errors in the manuscripts, he is confident that the “core concept” of STAP will be “verified by the RIKEN as well as independently by others”.
For many stem-cell researchers, the most shocking part of the STAP controversy was the involvement of Niwa, Sasai and Wakayama in such troubled work. “Co-authors of a paper like that should have been certain that they can reproduce results independently and in this case they should share responsibility,” says Davor Solter, a developmental and stem-cell biologist at the Institute of Medical Biology in Singapore. Wakayama takes the blame for not making more effort to check Obokata’s work, such as looking at her notebooks, which the investigative committee found to be alarmingly disorganized. Others sympathize with the researchers, who themselves were duped — whether through negligence or intention — by a junior colleague. “There has to be control, but also trust in science, otherwise the system breaks down completely,” says Maria Leptin, a molecular biologist and director of EMBO. “I cannot watch over every step while they are pipetting. That’s not the point.”
But in addition to lax oversight, Janet Rossant, a stem-cell researcher at the Hospital for Sick Children in Toronto, Canada, and the outgoing head of the International Society for Stem Cell Research, points to “poor reviewing and editing by Nature, who were also too ready to publish without verification”. Campbell disagrees. “Nature did not let down its guard,” he says.
Some say that the journal should publish reviewers’ comments to clarify the process. Campbell says that the publication of referees’ comments has been considered, but that the disadvantages — which include potential misinterpretations and the desire of many referees to keep their comments confidential — have prevented the journal from embracing this.
“We have to accept that where there is research, there will be research misconduct,” says Paul Taylor, a research-integrity adviser at the University of Melbourne, Australia. Efforts by institutions to train researchers and improve data-management infrastructure might help, “but no policy, no education or training, no administrative requirement, is going to stop misconduct”.
Taylor adds that the focus should be on how an institution responds. In that sense, he says the STAP problem seems to have been a success. RIKEN has acknowledged flaws in its data management and exaggeration in its press release for the STAP papers. Taylor says that its response has been fast, effective and transparent. In the midst of the investigation into Obokata’s work, Noyori instructed all RIKEN labs to review their published work — totalling tens of thousands of papers — for similar types of errors.
Among stem-cell researchers, STAP has become another cautionary tale to add to Hwang’s, with its own set of lessons. For Loring, the story stresses the importance of good record-keeping and the need to enter collaborations with caution. “I lecture my lab members that being an author carries responsibility for the validity of all of the work in the paper. I really try to live by that.” She says that she has removed her name from authorship lists in cases when she could not vouch for the quality of a manuscript.
But for many it is a lesson hard-learned, once again. “Reputation in science is everything,” says Trounson, in a statement that applies no less to journals and institutions than individual scientists. “Once gone, it’s extremely hard to get back.” ■
South Korean protesters place a defaced portrait of Japanese Prime Minister Shinzo Abe next to a statue of a South Korean teenage girl during an rally at the Japanese Embassy in Seoul on April 1. (Jung Yeon-Je/AFP/Getty Images)